1. Sasai, M., H. Oshiumi, K. Funami, M. Matsumoto, and T. Seya. 2010. Direct binding of TRAF2 and TRAF6 to TICAM-1/TRIF adaptor of the Toll-like receptor 3/4 pathway. Molec. Immunol. 47: 1283-1291.
  2. Kubota, N., T. Ebihara, M. Matsumoto, S. Gando, and T. Seya. 2010. IL-6 and interferon-alpha induced by polyI:C-stimulated bone-marrow-derived dendritic cells regulate peripheral expansion of regulatory T cells. Biochem. Biophys. Res. Commun. 391: 1421-1426.
  3. Hirata, N., Y. Yanagawa, M. Satoh, H. Ogura, T. Ebihara, M. Noguchi, M. Matsumoto, H. Togashi, T. Seya, K. Ono?, and K. Iwabuchi. 2010. Dendritic cell-derived TNF-a is responsible for development of IL-10-producing CD4+ T cells.Cell. Immunol. 261: 37-41.
  4. Oshiumi, H., K. Sakai, M. Matsumoto, and T. Seya. 2010. DEAD/H BOX 3 (DDX3) helicase binds the RIG-I adaptor IPS-1 to up-regulate IFN-beta inducing potential. Eur. J. Immunol. 40: 940-948.
  5. Akazawa, T., N. Inoue, H. Shime, K. Sugiura, K. Kodama, M. Matsumoto, and T. Seya. 2010. Adjuvant engineering for cancer immunotherapy: development of a synthetic TLR2 ligand with increased cell adhesion. Cancer Sci. 101: 1596-1603.
  6. Kasamatsu, J., H. Oshiumi, M. Matsumoto, Kasahara, and T. Seya. 2010. Phylogenetic and expression analysis of Lamprey Toll-like receptors. Dev. Comp. Immunol. 34: 855-865.
  7. Azuma, M., R. Sawahata, Y. Akao, T. Ebihara, S. Yamazaki, M. Matsumoto, M. Hashimoto, K. Fukase, Y. Fujimoto, and T. Seya. 2010. The peptide sequence of diacyl lipopeptides determines dendritic cell TLR2-mediated NK activation. PLoS ONE 5: e12550.
  8. Tatematsu, M., A. Ishii, M. Horiuchi, H. Oshiumi, F. Inagaki, T. Seya, and M. Matsumoto. 2010. Molecular mechanism for activation of Toll/IL-1 receptor domain-containing adaptor molecule-1 (TICAM-1). J. Biol. Chem. 285: 20128-20136.
  9. Ebihara, T., M. Azuma, H. Oshiumi, J. Kasamatsu, K. Iwabuchi, K. Matsumoto, H. Saito, T. Taniguchi, M. Matsumoto, and T. Seya. 2010. Identification of a polyI:C-inducible membrane protein, that participates in dendritic cell-mediated natural killer cell activation. J. Exp. Med. 207: 2675-2687.
  10. Ehira, N., H. Oshiumi, M. Matsumoto, T. Kondo, M. Asaka and T. Seya. 2010. An embryo-specific expressing TGF-beta family protein, growth-differentiation factor 3 (GDF3), augments progression of B16 melanoma. J. Exp. Clin. Cancer Res. 29: 135.
  11. Oshiumi, H., H. Mori, M. Ikeda, N. Kato, M. Matsumoto, O. Takeuchi, S. Akira, K. Shimotohno, and T. Seya. 2010. Hepatitis C virus (HCV) core protein promotes viral replication by abrogating IFN-b-inducing function of DEAD/H BOX 3 (DDX3) helicase. PLoS ONE. 5: e14258.
  12. Oshiumi, H., M. Miyashita, N. Inoue, M. Okabe, M. Matsumoto, and T. Seya. 2010. Essential role of Riplet in RIG-I-dependent antiviral innate immune responses. Cell host microbe. 8: 496-509.
  13. Seya, T., H. Shime, T. Ebihara, H. Oshiumi, and M. Matsumoto. 2010. Pattern-recognition receptors of innate immunity and their application to tumor immunotherapy. Cancer Sci. 101: 313-320 (review).
  14. Seya, T., 2010. Innate immunity and vaccine. Vaccine 28: 8041-8042. (preface).


  1. Seya T., and K. Miyake. 2009. Toll-like receptor.Encyclopedia of Molecular Medicine(Nature Publishing Group) Ed. by T. E. Creighton. John Wiley & Sons, Inc. New York, NY a0004028. www.els.net.
  2. Watanabe, T., K. Ito, M. Matsumoto, T. Seya, K. Hatakeyama, S. Nishikawa, T. Hasegawa, and K. Fukuda. 2009. Isolation and Characterization of RNA aptamers specific for the human Toll-like receptor 3 ectodomain. Viva Origino. 37: 10-18.
  3. Oshiumi, H., M. Matsumoto, S. Hatakeyama, and T. Seya. 2009. Riplet/RNF135, a RING-finger protein, ubiquitinates RIG-I to promote interferon-b induction during the early phase of viral infection. J. Biol. Chem. 284: 807-817.
  4. Wu, J. D., C. L. Atteridge, X. J. Wang, T. Seya, and S. R. Plymate. 2009. Obstructing shedding of the immune stimulatory MHC class I chain-related gene B prevents tumor formation. Clin. Cancer Res. 15: 632-640.
  5. Oshiumi, H., Y. Suzuki, M. Matsumoto, and T. Seya. 2009. Regulator of Complement Activation (RCA) gene cluster in Xenopus tropicalis. Immunogenetics 61:371-384.
  6. Akao, Y., T. Ebihara, H. Masuda, Y. Saeki, K. Hazeki, O. Hazeki, M. Matsumoto, and T. Seya. 2009. Enhancement of antitumor natural killer cell activation by orally administered Spirulina extract in mice. Cancer Sci. 100:1494-1501.
  7. Fujimoto, Y., M. Hashimoto, M. Furuyashiki, M. Katsumoto, T. Seya, Y. Suda, andK. Fukase. 2009. Innate Immunostimulatory lipopepitdes of Staphylococcus aureus as TLR2 ligands; Prediction with mRNA expression, chemical synthesis and immunostimulatory activities.Chem. Biol. Chem. 10: 2311-2315.
  8. Yasukawa, K., H. Oshiumi, M. Takeda, Y. Yanagi, T. Seya, S. Kawabata and T. Koshiba. 2009. Mitofusin 2, a mitochondrial fusion mediator, acts as a negative regulator for mitochondrial antiviral signaling. Science Signaling. 2(84): ra47.
  9. Kodama, K., M. Higashiyama, K. Takami, K. Oda, J. Okami, J. Maeda, T. Akazawa, M. Matsumoto, T. Seya, M. Wada, A. Hayashi, and K. Toyoshima. 2009. Innate immune therapy with a BCG cell wall skeleton after radical surgery for non-small cell lung cancer: a case-control study. Surgery Today. 39: 194-200.
  10. Takaki, H., H. Oshiumi, T. Kawanishi, M. Sasai, M. Matsumoto, and T. Seya. 2009. Cytoplasmic oligomerized TICAM-1 recruits nuclear BS69 to enhance type I interferon induction.Eur. J. Immunol.39: 3469-3476.
  11. Iwakiri. D.,L. Zhou, M. Samanta, M. Matsumoto, T. Ebihara, T. Seya, S. Imai, M. Fujieda, K. Kawa, and K. Takada. 2009. Epstein-Barr virus (EBV)-encoded small RNA is released from EBV-infected cells and activates signaling from Toll-like receptor 3. J. Exp. Med. 206: 2091-2099.
  12. Seya, T., M. Matsumoto, T. Ebihara, and H. Oshiumi. 2009. Functional evolution of the TICAM-1 (TRIF) pathway for extrinsic RNA sensing. Immunol. Rev. 227: 44-53 (review).
  13. Seya, T., and M. Matsumoto. 2009. The extrinsic RNA-sensing pathway for adjuvant immunotherapy for cancer. Cancer Immunol. Immunother. 58:1175- 1184 (review).
  14. Ebihara, T. M. Matsumoto, and T. Seya. 2009. Dendritic cell/NK cell interaction in RNA virus infection. Curr. Immunol. Rev. 5: 200-207 (review).


  1. Higuchi, M., A. Matsuo, M. Shingai, K. Funami, A. Ishii, H. Oshiumi, M. Matsumoto, and T. Seya. 2008. Combinational recognition of bacterial lipoproteins and peptidoglycan by chicken Toll-like receptor 2 subfamily. Dev. Comp. Immunol. 32: 147-155.
  2. Bas, S., L. Neff, M. Vuillet, U. Spenato, T. Seya, M. Matsumoto, and C. Gabay. 2008. The proinflammatory cytokine response to Chlamydia trachomatis elementary bodies in human macrophages is partly mediated by a lipoprotein, the macrophage infectivity potentiator, through TLR2/TLR1/TLR6 and CD14. J. Immunol. 180: 1158-1168.   
  3. Shime, H., M. Yabu, T. Akazawa, K. Kodama, M. Matsumoto, T. Seya and N. Inoue. 2008. Tumor-secreted lactic acid promotes IL-23-IL-17 proinflammatory pathway. J. Immunol. 180: 7175-7183.   
  4. Matsuo, A., H. Oshiumi, T. Tsujita, H. Mitani,H. Kasai, M. Yoshimizu, Matsumoto, and T. Seya. 2008. Teleost TLR22 recognizes RNA duplex to induce IFN and protect cells from birnaviruses. J. Immunol. 181: 3474-3485.
  5. Shingai, M., M. Azuma, T. Ebihara, M. Sasai, K. Funami, M. Ayata, H. Ogura, H. Tsutsumi, M. Matsumoto, and T. Seya. 2008. Soluble G protein of respiratory syncytial virus inhibts Toll-like receptor 3/4-mediated IFN-beta induction. Int. Immunol. .20: 1169-1180.
  6. Nakamura, M., K. Funami, A. Komori, T. Yokoyama, Y. Aiba, A. Araki, Y. Takii, M. Ito, M. Matsuyama, M. Koyabu, K. Migita, K. Taniguchi, H. Fujioka, H. Yatsuhashi, M. Matsumoto, H. Ishibashi, and T. Seya. 2008. Increased expression of Toll-Like Receptor3 in intrahepatic biliary epithelial cells at sites of ductular reaction in diseased livers. Hepatology International. 2: 222-230.
  7. Ebihara, T., M. Shingai, M. Matsumoto, T. Wakita, and T. Seya. 2008. Hepatitis C virus (HCV)-infected hepatocytes extrinsically modulate dendritic cell maturation to activate T cells and NK cells.Hepatology. 48: 48-58.
  8. Funami K., M. Sasai, H. Oshiumi, T. Seya, and M. Matsumoto. 2008. Homo-oligomerization is essential for Toll/IL-1 receptor domain containing adaptor molecule-1-mediated NF-kappaB and interferon regulatory factor-3 activation. J. Biol. Chem.283: 18283-18291
  9. Fukuda, K., T. Watanabe, T. Tokisue, T. Tsujita, S. Nishikawa, T. Hasegawa, T. Seya, and M. Matsumoto. 2008. Modulation of Double-stranded RNA Recognition by the N-terminal Histidine-rich Region of the Human Toll-like Receptor 3. J.Biol.Chem. 283: 22787-22794.   
  10. Hirata, N., Y. Yanagawa, T. Ebihara, T. Seya, S. Uematsu, S. Akira, F. Hayashi, K. Iwabuchi, and K. Onoe´. 2008. Selective synergy in anti-inflammatory cytokine production upon cooperated signaling via TLR4 and TLR2 in murine conventional dendritic cells. Mol. Immunol. 45: 2734-2742.
  11. Itoh, K., A. Watanabe, K. Funami, T. Seya, and M. Matsumoto, 2008. The clathrin-mediated endocytic pathway participates in dsRNA-induced IFN-b production. J. Immunol. 181: 5522-5529.
  12. Matsumoto, M., and T. Seya. 2008. TLR3 : interferon induction by double-stranded RNA including poly(I:C). Adv. Drug Deliv. Rev. 60: 805-812.
  13. Seya, T. 2008. Preface: Toll-like receptor and pattern sensing fore voking immune response. Adv. Drug Deliv. Rev. 60: 779-781.
  14. Oshiumi, H., A. Matsuo, M. Matsumoto, and T. Seya. 2008. Pan-vertebrate Toll-like receptors during evolution.Curr. Genomics 9: 488-493 (Review).


  1. Ishii, A., H. Sawa, T. Tsujita, K. Shida, A. Matsuo, M. Matsumoto, and T. Seya. 2007. Lamprey Toll-like receptors with properties distinct from those of variable lymphocyte receptors. J. Immunol. 178: 397-406.
  2. Niimi, K. K. Asano, Y. Shiraishi, T. Nakajima, M. Wakaki, J. Kagyo, T. Takihara, Y. Suzuki, K. Fukunaga, T. Shiomi, T. Oguma1, K. Sayama, K. Yamaguchi, Y. Natori, M. Matsumoto, T. Seya, and A. Ishizaka. 2007. Toll-like receptor 3-mediated eotaxin-1 release from human bronchial smooth muscle cells. J. Immunol. 178: 489-495.
  3. Akazawa T., M. Okuno, Y. Okuda, K. Tsujimura, T. Takahashi, M. Ikawa, M. Okabe, T. Ebihara, M, Shingai, N. Inoue, M. Tanaka-Okamoto, H. Ishizaki, J. Miyoshi, M. Matsumoto, and T. Seya. 2006. Antitumor NK activation induced by the Toll-like receptor3-TICAM-1 (TRIF) pathway in myeloid dendritic cells. Proc. Natl. Acad. Sci. USA. 104: 252-257.
  4. Sukenobu, N., K. Hazeki, K. Yoshikawa, T. Yamamoto, U. Kikkawa, M. Matsumoto, T. Seya, and O. Hazeki. 2006. Protein kinase Cd associates TIRAP/Mal and regulates NF-kB and mitogen-activated protein kinases in Toll-like receptor signaling. Molec. Immunol. 44: 2257-2264.
  5. Azuma M., A. Matsuo, Y. Fujimoto, K. Fukase, K. Hazeki, O. Hazeki, M. Matsumoto, and T. Seya. 2007. Inhibition of lipid A-mediated type I interferon induction by Bactericidal/peameability-increasing protein (BPI). Biochem. Biophys. Res. Commun. 354: 574-578.
  6. Shingai, M., T. Ebihara, N. A. Begum, A. Kato,, T. Honma, K. Matsumoto, H. Saito, H. Ogura, M. Matsumoto, and T Seya. 2007. Differential I interferon (IFN) inducing abilities of wild-type vs. vaccine strains of measles virus. J. Immunol. 179: 6123-6133.
  7. Ebihara, T., H. Masuda, T. Akazawa, M. Shingai, H. Kikuta, T. Ariga, M. Matsumoto, and T. Seya. 2007. NKG2D ligands are induced on human dendritic cells by TLR ligand stimulation and RNA virus infection. Int. Immunol.19: 1145-1155 .
  8. Ishii, A., M. Kawasaki, M. Matsumoto, S. Tochinai, and T. Seya. 2007. Phylogenetic and expression analysis of amphibian Xenopus Toll-like receptors. Immunogenetics 59: 281-293.
  9. Takada, E., S. Okahira, M. Sasai, K. Funami, T. Seya, and M. Matsumoto. 2007. C-terminal LRRs of human Toll-like receptor 3 control receptor dimerization and signal transmission. Molec. Immunol 581: 3334-3340
  10. Akazawa T., M. Ikawa, M. Okabe, T. Ebihara, M, Shingai, N. Inoue, M. Matsumoto, and T. Seya. 2007. Tumor regression induced by adoptive transfer of MyD88-transfected dendritic cells in a mouse model. FEBS lett. 581: 3334-3340
  11. Yoshioka, M., N. Fukuishi, S. Iriguchi, K. Ohsaki, H. Yamanobe, A. Inukai, D. Kurihara, N. Imajo, Y. Yasui, N. Matsui, T. Tsujita, A. Ishii, T. Seya, M. Takahama, and M. Akagi. 2007. Lipoteichoic acid down-regulates Fce receptor I expression on human mast cells through Toll-like receptor 2. J. Aller. Clin. Immunol. 120: 452-461
  12. Shiratori, I., Y.Suzuki, H.Osiumi, N. A. Begum, T. Ebihara, M. Matsumoto, K. Hazeki, K. Kodama, Y. Kashiwazaki, and T. Seya. 2007. Recombinant IL-12/IL-18 therapy in a guinea pig hepatoma cell implanted model.Canser Sci. 98: 1936-1942.
  13. Funami K., M. Sasai, Y. Ohba, H. Oshiumi, T. Seya, and M. Matsumoto. 2007. Spatiotemporal mobilization of TICAM-1 in response to dsRNA.J Immunol. 179: 6867-6782.
  14. Ayata, M., M. Shingai, X. Ning, M. Matsumoto, T. Seya, S. Otani, T. Seto, S. Oshimoto, and H. Ogura. 2007. Effect of the alterations in the fusion protein of measles virus isolated from brains of patients with subacute sclerosing panencephalitis on syncytium formation.Virus Res. 130: 260-268.
  15. Yamazaki, K., k. Suzuki, E. Yamada, T. Yamada, F. Takeshita, M. Matsumoto, T. Mitsuhashi, T. Obara, K. Takano, and K.Sato. 2007. Suppression of iodide uptake and thyroid hormone synthesis with stimulation of the type I interferon system by double-stranded RNA (dsRNA) in cultured human thyroid follicles. Endocrinology. 148: 3226-3235
  16. Seya, T., M. Matsumoto, T. Ebihara, and T. Akazawa. 2007. NK-activating dendritic cells are elicited by stimulation with TLR3 ligand. Hirosaki Med. J. 59 (suppl): 43-51.


  1. Tsujita, T., A. Ishii, H. Tsukada, M. Matsumoto, F-S. Che, and T. Seya. 2006. Fish soluble Toll-like receptor (TLR)5 amplifies human TLR5 response via physical binding to flagellin. Vaccine 24: 2193-2199.
  2. Ii, M., N. Matsunaga, K. Hazeki, K. Nakamura, K. Takashima, T. Seya, O. Hazeki, T. Kitazaki, and Y. Iizawa. 2006. A novel cyclohexene derivative, TAK-242, selectively inhibits Toll-like receptor 4-mediated cytokine production through suppression of intracellular signaling. Mol Pharmacol. 69: 1288-95.
  3. Muromoto, R., K. Okabe, M. Fujimuro, K. Sugiyama, H. Yokosawa, T. Seya, and T. Matsuda. 2006. Physical and functional interactions between STAT3 and Kaposi’s sarcoma-associated herpesvirus-encoded LANA. FEBS Lett. 580: 93-98.
  4. Sakurai, F., S. Murakami, K. Kawabata, N. Okada, A. Yamamoto, T. Seya, T. Hayakawa, and H. Mizuguchi. 2006. Crucial role of the short consensus repeats 1 and 2, not the cytoplasmic domain, of human CD46 on infection of subgroup B adenovirus serotype 35. J. Control Release 113: 271-278.
  5. Wrackmeyer, U., G. H. Hansen, T. Seya, and E. M. Danielsen. 2006. Intelectin: a novel lipid reft-associated protein in the enterocyte brush border. Biochemistry 45: 9188-9197.
  6. Sasai, M., M. Shingai, K. Funami, M. Yoneyama, T. Fujita, M. Matsumoto, and T. Seya. 2006. NAP1 (NAK-associated protein 1) participates in both the TLR3 and the cytoplasmic pathways in type I interferon induction. J. Immunol. 177: 8676-8683.
  7. Sasai, M., M. Matsumoto, and T. Seya. 2006. The kinase complex responsible for IRF-3-mediated IFN-{beta} production in myeloid dendritic cells (mDC).. J. Biochem (Tokyo) 139: 171-175 (review).
  8. Seya T., T. Akazawa, T. Tsujita, and M. Matsumoto. 2006. Role of Toll-like receptors in adjuvant-augmented immune therapies. eCAM. 3: 31-38 (review)